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pgl3 control firefly luciferase vector  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co pgl3 control firefly luciferase vector
    Pgl3 Control Firefly Luciferase Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 98/100, based on 1808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+vector/Dual+Luciferase+Reporter+Assay+Kit/pmc12710952-153-13-17
    Average 98 stars, based on 1808 article reviews
    pgl3 control firefly luciferase vector - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Synthesized:

    Article Title: Efficient Modulation of Exon Skipping via Antisense Circular RNAs
    Article Snippet: To construct a vector expressing linear AS-RNA, the PGL3 vector (Addgene #107721) was linearized by BsaI (NEB #R3733) and EcoRI (NEB #R3101). .. Antisense sequences of less than 50 base pairs (bp) were chemically synthesized, annealed, and ligated to the linearized PGL3 vector by T4 DNA ligase (Vazyme #C301). .. Antisense sequences greater than 100 bp were amplified by PCR and ligated to the linearized PGL3 vector using the ClonExpress II One Step Cloning Kit (Vazyme #C112).

    Plasmid Preparation:

    Article Title: Efficient Modulation of Exon Skipping via Antisense Circular RNAs
    Article Snippet: To construct a vector expressing linear AS-RNA, the PGL3 vector (Addgene #107721) was linearized by BsaI (NEB #R3733) and EcoRI (NEB #R3101). .. Antisense sequences of less than 50 base pairs (bp) were chemically synthesized, annealed, and ligated to the linearized PGL3 vector by T4 DNA ligase (Vazyme #C301). .. Antisense sequences greater than 100 bp were amplified by PCR and ligated to the linearized PGL3 vector using the ClonExpress II One Step Cloning Kit (Vazyme #C112).



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